Journal: Genetics Research

Article Title: Identification of ECI2 as Potential Prognostic Biomarkers Based on a Fatty Acid Metabolism-Related Gene Model in Clear Cell Renal Cell Carcinoma

doi: 10.1155/genr/2237539

Figure Lengend Snippet: Screening of differentially expressed genes related to fatty acid metabolism. (a) Single-cell data clustering ump plot of GSE152938 . (b) Differential expression of fatty acid metabolism gene set between tumor and adjacent tissues of ccRCC. (c) Differential expression of fatty acid metabolism gene set among different cell types. (d) Combining transcriptomics, proteomics, and single-cell genomics to screen FA-DEGs. (e) Protein interaction networks of 10 FA-DEGs using GENEMIA database. (f) Differential expression of 10 FA-DEGs in TCGA transcriptome. Data were shown as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Human-derived ccRCC cell lines (OS-RC-2, 769P, 786-O) and normal renal tubular epithelial cell line (HK-2) were used, all of which were purchased from ServiceBio.

Techniques: Quantitative Proteomics

Journal: Genetics Research

Article Title: Identification of ECI2 as Potential Prognostic Biomarkers Based on a Fatty Acid Metabolism-Related Gene Model in Clear Cell Renal Cell Carcinoma

doi: 10.1155/genr/2237539

Figure Lengend Snippet: Prognostic model related to fatty acid metabolism. (a) Cross-validation plot for the penalty term. (b) Plots for LASSO expression coefficients of the FA-DEGs. (c) Risk score, survival time, and survival status of ccRCC patients in TCGA. (d) The KM survival curve distribution of this risk model in ccRCC patients, where log rank is used to test between different groups. (e) Calibration curve of fatty acid metabolism–related risk model. (f) Screening key FA-DEGs using Cytoscape.

Article Snippet: Human-derived ccRCC cell lines (OS-RC-2, 769P, 786-O) and normal renal tubular epithelial cell line (HK-2) were used, all of which were purchased from ServiceBio.

Techniques: Biomarker Discovery, Expressing

Journal: Genetics Research

Article Title: Identification of ECI2 as Potential Prognostic Biomarkers Based on a Fatty Acid Metabolism-Related Gene Model in Clear Cell Renal Cell Carcinoma

doi: 10.1155/genr/2237539

Figure Lengend Snippet: Analysis of ECI2 expression levels and its correlation with clinical characteristics and prognosis. (a) ECI2 expression in paired samples in ccRCC cohort of TCGA. (b) ROC analysis of ECI2 in ccRCC cohort diagnosis. (c) Differences protein expression levels of ECI2 in CPTAC. (d) Differences phosphorylated protein expression levels of ECI2 in CPTAC. (e) Analysis of the correlation between ECI2 expression and ccRCC patients' overall survival. (f) Analysis of the correlation between ECI2 expression and ccRCC patients' disease-specific survival. (g) Analysis of the correlation between ECI2 expression and ccRCC patients' progression-free survival. (h) Correlation analysis between the expression level of ECI2 and the pathological N stage of ccRCC patients. (i) Correlation analysis between the expression level of ECI2 histologic stage of ccRCC patients. (j) Analysis of methylation levels of ECI2 at various points. (k) Correlation analysis between methylation level and mRNA expression level of ECI2 at cg11647493 site. Data were shown as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Human-derived ccRCC cell lines (OS-RC-2, 769P, 786-O) and normal renal tubular epithelial cell line (HK-2) were used, all of which were purchased from ServiceBio.

Techniques: Expressing, Biomarker Discovery, Methylation

Journal: Genetics Research

Article Title: Identification of ECI2 as Potential Prognostic Biomarkers Based on a Fatty Acid Metabolism-Related Gene Model in Clear Cell Renal Cell Carcinoma

doi: 10.1155/genr/2237539

Figure Lengend Snippet: Expression validation of ECI2 in clinical samples and cell lines. (a) Differences in protein expression levels of ECI2 in ccRCC tumor tissue and adjacent tissues ( n = 8). (b) Differences in mRNA expression levels of ECI2 in ccRCC tumor tissue and adjacent tissues ( n = 8). (c) Immunohistochemical staining of ccRCC tumor tissue and adjacent tissues ( n = 40). Scale bars: 200 μm. (d) Differences in protein expression levels of ECI2 in ccRCC cell lines. (e) Differences in mRNA expression levels of ECI2 in ccRCC cell lines. (f) Immunofluorescence staining confirms the subcellular localization of ECI2 (using FITC to label ECI2 and CY3 to label mitochondrial protein TOM20). Data were shown as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Human-derived ccRCC cell lines (OS-RC-2, 769P, 786-O) and normal renal tubular epithelial cell line (HK-2) were used, all of which were purchased from ServiceBio.

Techniques: Expressing, Biomarker Discovery, Immunohistochemical staining, Staining, Immunofluorescence

Journal: Genetics Research

Article Title: Identification of ECI2 as Potential Prognostic Biomarkers Based on a Fatty Acid Metabolism-Related Gene Model in Clear Cell Renal Cell Carcinoma

doi: 10.1155/genr/2237539

Figure Lengend Snippet: Overexpression of ECI2 inhibits the proliferation and migration of ccRCC cells. (a) Differences in protein and mRNA expression levels of TBRG4 after lentiviral transfection into 786O. (b) The proliferation of 786O was examined by cell counts. (c) Colony formation assay was employed to evaluate the proliferation of 786O. (d) Wound healing assay measured the motor ability of 786O. (e) The transwell assay detected the migration of 786O. (f) Calculation of IC 50 differences after 48 h of treatment with different concentrations of oxaliplatin. (g) Western blot detection of protein expression differences between BCL2 and Caspase3 after 48 h of IC 50 oxaliplatin treatment. Data were shown as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Human-derived ccRCC cell lines (OS-RC-2, 769P, 786-O) and normal renal tubular epithelial cell line (HK-2) were used, all of which were purchased from ServiceBio.

Techniques: Over Expression, Migration, Expressing, Transfection, Colony Assay, Wound Healing Assay, Transwell Assay, Western Blot

Journal: Frontiers in Endocrinology

Article Title: Identification of a novel prognostic and therapeutic prediction model in clear cell renal carcinoma based on Renin-angiotensin system related genes

doi: 10.3389/fendo.2025.1521940

Figure Lengend Snippet: The role of SLC6A19 in ccRCC; (A) Univariate Cox regression for SLC6A19, SLC6A12 and SMIM24. (B) Differential expression of SLC6A19 between tumor and normal tissues in TCGA,GSE53757; (C) Differential expression of SLC6A19 in various clinical stages; (D) Immunohistochemical result from the HPA database showing SLC6A19 expression in normal tissue; (E) Immunohistochemical result from the HPA database showing SLC6A19 expression in ccRCC tissue; (F) Transwell assay showing the impact of SLC6A19 to invasive ability of 786O and A498 cell lines; (G) CCK8 assay showing the impact of SLC6A19 to proliferation of 786O and A498 cell lines. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001. ns, not significant.

Article Snippet: The 786O and A498 cancer cell lines were purchased from CellSource China.

Techniques: Quantitative Proteomics, Immunohistochemical staining, Expressing, Transwell Assay, CCK-8 Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: The LncRNA DUXAP10 Could Function as a Promising Oncogene in Human Cancer

doi: 10.3389/fcell.2022.832388

Figure Lengend Snippet: Functions and mechanisms of DUXAP10 in cancers.

Article Snippet: High expression of DUXAP10 was observed in 18 RCC specimens collected from the Urology Department of Peking University Shougang Hospital and 786-O and A498 cell lines in comparison with adjacent normal tissues and normal kidney epithelial cells (HKCs).

Techniques: Migration, Transformation Assay